Informatics Publishing Limited

K. Natarajan ¹, S. Ramanathan ¹, R. Sathish ¹, C. Amudhadevi ¹

Affiliations
1 Department of Pharmaceutical Biotechnology, Ultra College of Pharmacy, Madurai, IN

Abstract

Many marine origin organic molecules entered clinical studies for anticancer therapy. Ascidians, a marine invertebrate animal draw attention of the researchers for its versatility as medicine and nutrition. In this study methylene chloride crude extract of Polyclinum madrasensis Sebastian (Ascidaciae) was tested for its in-vitro anticancer property on HeLa cell lines (cervical cancer cell lines) using MTT assay. Results revealed that the extract exhibits cytotoxic effect on the cell lines used (IC 50- 0.23mg/ml). Extract was further elucidated using chemical and spectral analysis (IR, HNMR), in which peptides were found to be the major constituent. As a conclusion, Polyclinum madrasensis is found to be a good source for drug lead component in cancer drug discovery process.

Keywords

Polyclinum madrasensis, PME, Cytotoxicity, HeLa.

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K. Natarajan ¹, S. Tamilarasi ¹, R. Sathish ¹, C. Amudhadevi ¹, T. Regupathi ¹

Affiliations
1 Ultra College of Pharmacy, Madurai, IN

Abstract

Different soil microbes were analyzed for their phosphate solubilizing capacity. The characterization of the microbes which exhibits phosphate solubilisation in preliminary screening reveals that the isolated strains were Pseudomonas aeroginosa, Pseudomonas flurosence, Bacillus subtilis, Bacillus cereus and E. coli. General parameters for determining phosphate solubilisation in PVK broth such as pH changes, estimation of phosphorus release in media and the phosphatase enzyme activity were performed. Investigation results showed high potency of the microbes to solubilize TCP. From the above tested organisms Pseudomonos pocess high efficiency to solubilize TCP followed by Bacillus and E.coli on tested all parameters. Biofertilizer was developed and nursery experiments were conducted with paddy crop. Parameters such as Germination of seed, Color of the crop and Height were analyzed. Biofertilizer treated crop displayed comparatively good results with non treated and chemical fertilizer treated crop. In conclusion Pseudomonas and Bacillus strains showed good phosphate solubilisation and can be utilized in biofertilizers.

Keywords

Phosphate Solubilization, PVK Broth, Pseudomonas, Bacillus.

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